Search results for "Internal ribosome entry site"
showing 4 items of 4 documents
Regulation of human inducible nitric oxide synthase expression by an upstream open reading frame.
2019
Abstract The human inducible nitric oxide synthase (iNOS) gene contains an upstream open reading frame (uORF) in its 5′-untranslated region (5′-UTR) implying a translational regulation of iNOS expression. Transfection experiments in human DLD-1 cells revealed that the uORF although translatable seems not to inhibit the translation start at the bona fide ATG. Our data clearly show that human iNOS translation is cap-dependent and that the 5′-UTR of the iNOS mRNA contains no internal ribosome entry site. Translation of the bona fide coding sequence is most likely mediated by a leaky scanning mechanism. The 5′-UTR is encoded by exon 1 and exon 2 of the iNOS gene with the uORF stop codon located…
Sequences in the 5′ Nontranslated Region of Hepatitis C Virus Required for RNA Replication
2001
ABSTRACT Sequences in the 5′ and 3′ termini of plus-strand RNA viruses harbor cis -acting elements important for efficient translation and replication. In case of the hepatitis C virus (HCV), a plus-strand RNA virus of the family Flaviviridae , a 341-nucleotide-long nontranslated region (NTR) is located at the 5′ end of the genome. This sequence contains an internal ribosome entry site (IRES) that is located downstream of an about 40-nucleotide-long sequence of unknown function. By using our recently developed HCV replicon system, we mapped and characterized the sequences in the 5′ NTR required for RNA replication. We show that deletions introduced into the 5′ terminal 40 nucleotides abolis…
460. MGMTP140K Vector Constructs for Efficient In Vivo Enrichment of Genetically Corrected Cells Following Hematopoietic Cell Gene Transfer
2006
MGMTP140K, a mutant of the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) resistant to the inhibitor O6-benzylguanine (BG), protects hematopoietic stem cells (HSC) from the toxicity of alkylating agents such as temozolomide (TMZ) and, therefore, allows efficient in vivo enrichment of transduced cells by BG/TMZ therapy. MGMTP140K has been proposed as a selection marker in gene therapy of monogeneic diseases. If used for this purpose, however, MGMTP140K expression in vectors containing internal ribosomal entry sites (IRES) would be from the compromised 3' position, as the 5' position will be required for the therapeutic gene. Thus, we have investigated SFFV/MESV-based vector…
IAP proteins as targets for drug development in oncology.
2013
The inhibitors of apoptosis (IAPs) constitute a family of proteins involved in the regulation of various cellular processes, including cell death, immune and inflammatory responses, cell proliferation, cell differentiation, and cell motility. There is accumulating evidence supporting IAP-targeting in tumors: IAPs regulate various cellular processes that contribute to tumor development, such as cell death, cell proliferation, and cell migration; their expression is increased in a number of human tumor samples, and IAP overexpression has been correlated with tumor growth, and poor prognosis or low response to treatment; and IAP expression can be rapidly induced in response to chemotherapy or …